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| Influence of“Kechuan Fang”on eosinophil apoptosis of lung tissue in guinea-pig with asthma |
| Hits 2164 Download times 2060 Received:July 20, 2004 |
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| DOI
10.11656/j.issn.1672-1519.2004.04.26 |
| Key Words
asthma;guinea-pig;eosinophils;Fas;bcl-2 |
| Author Name | Affiliation | | LIU Gui-ying | The First Affiliated Hospital to Tianjin University of TCM, Tianjin300193, China | | LIU Yu-qing | The First Affiliated Hospital to Tianjin University of TCM, Tianjin300193, China | | LV Ying | The First Affiliated Hospital to Tianjin University of TCM, Tianjin300193, China | | 尹新中 | The First Affiliated Hospital to Tianjin University of TCM, Tianjin300193, China | | 刘兰萍 | The First Affiliated Hospital to Tianjin University of TCM, Tianjin300193, China | | 张慧琪 | The First Affiliated Hospital to Tianjin University of TCM, Tianjin300193, China | | 朱振刚 | The First Affiliated Hospital to Tianjin University of TCM, Tianjin300193, China | | 刘旻 | The First Affiliated Hospital to Tianjin University of TCM, Tianjin300193, China | | 常力 | The First Affiliated Hospital to Tianjin University of TCM, Tianjin300193, China |
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| Abstract
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| [Objective] To investigate the effect of the“Kechuan Fang”on eosinophils apoptosis of lung tissue in guinea-pig with asthma.[Method] Guinea pigs were randomly divided into four groups,asthma model group,prednisone group,“Kechuang Fang”group and normal control group.Eosinophils were isolated from the bronchovesicular lavage.Eosinophils and eosinophils apoptosis counts in every group were measured with biochemistry and immunohistochemistry.Fas-mRNA and bcl2-mRNA expression were determined by means of in situ blotting.The results were analyzed using colour- pathological image analysis system.[Results] The eosinophils counts and infiltration in“Kechuang Fang”group were significantly lower than those in both asthma model group and prednisone group,but eosinophils apoptosis counts were higher significantly than those in that two groups.Eosinophils Fas-mRNA expression of lung in the asthma guinea-pig in“Kechuang Fang”group increased more significant than that of others,but eosinophils bcl2-mRNA expression decreased more significant than that of both the asthma model group and prednisone group.[Conclusion] The“Kechuang Fang”can reduce eosinophils infiltration in asthma guinea-pig lung tissue,promote eosinophils apoptosis-gene Fas expression,and induce eosinophils apoptosis. |
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