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| Content determination of Rhein in Zhikexiao Capsule by HPLC |
| Hits 1197 Download times 1460 Received:December 26, 2003 |
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| DOI
10.11656/j.issn.1672-1519.2004.05.30 |
| Key Words
Zhekexiao Capsule;Rhein;content determination;HPLC |
| Author Name | Affiliation | | GAO Jing | Tianjin Academy of Pharmaceutical Research, Tianjin 300193, China | | CHEN Zhi-juan | 天津中医学院, 天津, 300193 | | ZHANG Shu-hua | Tianjin Academy of Pharmaceutical Research, Tianjin 300193, China |
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| Abstract
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| [Objective] To establish a method for the content determination of Rhein in Zhikexiao Capsule. [Methods] A simple HPLC method for determination of Rhein was established.The determination was carried out on ODS column with mobile phase of methanol-water-acetic acid(83:17:0.2)and detection wave-length at 254nm. [Result] The Rhein sample size showed a good linear relationship at the range of 0.1～0.5μg and correlation coefficient was 0.9998.The average re-covery rate of the added sample was 99.97%.RSD was 1.91%. [Conclusion] The method is simple,accurate,reproducibe and can be used for content determination of preparation and medicinal materials. |
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