|
| Effect of Salvianolic Acid B Preconditioning on Expression of Heat Shock Protein 70mRNA in Cardiac Microvascular Endothelial Cells Injured by Hypoxia and Reoxygenation |
| Hits 1417 Download times 1605 Received:August 12, 2004 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2004.06.06 |
| Key Words
salvianolic acid B;preconditiong;heat shock protein70;hypoxia and reoxygenation |
| Author Name | Affiliation | | GUO Li-ping | The First Hospital affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | ZHANG Meng | 天津中医学院 天津300193 | | DU Rong | 天津中医学院 天津300193 | | 高秀梅 | 天津中医学院 天津300193 | | 张伯礼 | 天津中医学院 天津300193 |
|
| Abstract
|
| [Objective] To explore whether the mechanism of the cellular protection of salvisnolic acid B (Sal B) preconditioning is relevant with the synthesis of the heat shock protein 70 (HSP70). [Methods] Technique of RT-PCR was adopted,and the impact of Sal B preconditioning on the expression of heat shock protein 70 (HSP70) mRNA in cardiac microvascular endothelial cells (CMEC) injured by hypoxia and reoxygenation was observed. [Results] HSP70 produced less expression in normal CMEC and its expression was increased significantly by hypoxia stimulating. Sal B preconditioning enhanced the expression of HSP70 mRNA and the effect was obviously superior to that of hypoxic preconditioning (HPC). During the delayed preconditioning protection, Sal B also could increase the HSP70 mRNA expression and enhance the effect of HPC. [Conclusion] Sal B preconditioning produces a protective effect on cells by inducing the expression of HSP70. |
|
