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Study on Quality Standard for Guzeng Mixture
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DOI   10.11656/j.issn.1672-1519.2004.06.28
Key Words   Guzeng Mixture;paeoniflorin;puerarin;psoralen;quality standard;TLC;resin-UV
Author NameAffiliation
GAO Su-qin Changzhou Hospital of Traditional Chinese Medicine, Changzhou 213002, China 
WU Yue-yun Changzhou Hospital of Traditional Chinese Medicine, Changzhou 213002, China 
JIN Ying 南京中医药大学 南京210029 
顾燕华 南京中医药大学 南京210029 
Abstract
    [Objective] To perfect the quality standard for Guzeng (bone-increasing)Mixture. [Methods] TLC was performed for the qualitative discrimination of the main Chinese Herbal cpmponents in Guzeng Mixture, including Radix Paeoniae Alba, Radix Puerariae, Fructus psoraleae and Herba epimedii. Epimedium glycoside, the effective content of epimedium herb was measured by Resin-UV method. [Results] Good spots separation of the four main herbs was obtained with significant contrasting. The calibration curve of epimedium glycoside was linear at range of 2.5-22.5μg/m (r=0.997). The average recovery rate was 95.5% (n=5,RSD= 1.52%). The precision degree of RSD was 1.93%. [Conclusion] The method is simple, accurate and reproducible. So it can be used for the quality control of this mixture.

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