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| Establishment of An in vitro BBB Model with Co-cultured Rat Brain Microvessel Endothelial Cells and Astrocytes |
| Hits 2022 Download times 3320 Received:January 24, 2005 |
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| DOI
10.11656/j.issn.1672-1519.2005.02.29 |
| Key Words
brain microvessel endothelial cells (BMEC);astrocyte (AC);co-culture;in vitro blood-brain barrier |
| Author Name | Affiliation | | HU Li-min | Tianjin University of TCM, Tianjin 300193, China | | FAN Xiang | Tianjin University of TCM, Tianjin 300193, China | | ZHANG Yan-jun | Tianjin University of TCM, Tianjin 300193, China | | 张伯礼 | Tianjin University of TCM, Tianjin 300193, China | | 梅建勋 | Tianjin University of TCM, Tianjin 300193, China | | 高秀梅 | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To establish an in vitro BBB model with co-cultured rat brain microvessel endothelial cells and astrocytes. [Methods] Rat brain microvessel endothelial cells (BMEC) and astrocytes (AC) were isolated and purified respectively and in vitro BBB model was established with a co-cultured method of BMEC and AC. The BMEC was inoculated on the inferior surface allowing their endfeet to contact with the basal surface of the endothelium or its basal lumina. Transendothelial electrical resistance (TEER) γ-glutamyl transpeptidase (γ-GT) and alkaline hosphatase (ALP) activity of the BBB immunohistochemistry staining of tight junction protein (ZO-1) were served as the evaluating criterion of the BBB model. [Results] Co-culture of astrocytes and brain microvessel endothelial cells could enhance the expression of the BBB-special enzymes. It could also increase the transendothelial electrical resistance. [Conclusion] The in vitro BBB model was established and can be used in the drug research. |
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