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| Determination of Hydrochloric Berberine in Burn Ⅱ Medicine by RP-HPLC |
| Hits 1422 Download times 1420 Received:October 08, 2004 |
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| DOI
10.11656/j.issn.1672-1519.2005.03.34 |
| Key Words
RP-HPLC;Burn Ⅱ medicine;hydrochloric berberine |
| Author Name | Affiliation | | QIN Hong-lin | Institute for Drug and Instrument Control, Beijing Military Region of PLA., Beijing 100071, China | | GAO Yue | 军事医学科学院放射医学研究所, 北京, 100850 | | LIU Li-li | 军事医学科学院放射医学研究所, 北京, 100850 |
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| Abstract
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| [Objective] To determine the content of hydrochloric Berberine in burn Ⅱ medicine. [Methods] Reverse-phase high performance liquid chromatography was applied with a C18 column(250 mm×4.6 mm,5 μm), taking 0.02 mol/L phosphate buffer-acetonitrile(68:32)as the mobile phase. The detective wavelength was at 270 nm. [Results] The mean recovery rate was 98.08% (RSD=1.97%, n=5). [Conclusion] The method is rapid, sensitive and accurate with reproducible results. |
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