|
| Inhibitory Effect and Mechanism of Runing Granule Preparation and Its Modyfied Prescription on Pulmonary Metastasis of Transplanted Tumou in Naked Mouse |
| Hits 1688 Download times 1694 Received:February 01, 2005 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2005.04.25 |
| Key Words
Runing Granule preparatiion;breast cancer;pulmonary metastasis;in vivo experimental study |
| Author Name | Affiliation | | XUE Xiao-hong | | | LIU Sheng | | | YANG Xin-Wei | | | 陆德铭 | |
|
| Abstract
|
| [Objective] To discuss the inhibitory effect and mechanism of Runing granule preparation and its modified prescription on pulmonary metastasis of transplanted tumor by in vivo experiment study.[Methods] BALB/c female naked mice were used.The animal models were established by transplanting highly metastatic MDA-MB-435 breast tumor cell.The mice were divided randomly into model group,Runing granule preparation group,warming kidney group,dispersing liver and promoting blood circulation group,tamoxifen(TAM) group and cyclophosphamide(CTX) group.Seven weeks after medication,the number of pulmonary metastasis was accounted by means of anatomic microscope.Slice of lung tissue was obtained to detect the express of VEGF,MMP-9 and TIMP-1 by Immunohistochemistry.[Result] The inhibitory effect on pulmonary metastasis of transplanted tumor were 46.36%,32.73%,18.2% in the Runing granule preparation group,warming kidney group,dispersing liver and promoting blood circulation group respectively.There wasn’t significant difference between Runing granule preparation group and CTX group(50.91%).The expression of VEGF was dropped in Runing granule preparation group and dispersing liver and promoting blood circulation group.The expression of TIMP-1mRNA was up-regulated in Runing Granule group and warming kidney group.The expression of MMP-9 was dropped in Runing granule preparation group and warming kidney group.The ratio of MMP-9 and TIMP-1 expression was obviously lower in warming kidney group.[Conclusion] The tumor-inhibitory effect of Runing granule preparation is better than its modified prescriptioin.The warming kidney group was better than dispersing liver and promoting blood circulation group in the inhibitory effect of metastasis effect.The Runing granule preparation,especially warming kidney group,may affect the occurrence of pulmonary metastasis through decreasing the ratio of MMP-9/TIMP-1. |
|
|
|
|
|
|
|
