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| Influence of Medicine-contained Serum of Jianpi Bushen Prescription on Spleen Cell Cycle of Mice cultured in Vitro |
| Hits 1587 Download times 1866 Received:August 12, 2005 |
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| DOI
10.11656/j.issn.1672-1519.2006.01.20 |
| Key Words
Jianpi Bushen Prescription;flowcytometry;cell cycle |
| Author Name | Affiliation | | ZHANG Yu-huan | Tianjin Changzheng Hospital, Tianjin 300021, China | | CHEN Bao-jiang | Tianjin University of TCM, Tianjin 300193, China | | WANG Ping | Tianjin Medical University, Tianjin 300070, China | | 冀英 | Tianjin Medical University, Tianjin 300070, China | | 焦振山 | Tianjin Changzheng Hospital, Tianjin 300021, China | | 李晓莉 | Tianjin Changzheng Hospital, Tianjin 300021, China |
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| Abstract
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| [Objective] To observe the effects of the medicine-contained serum of Jianpi Bushen Prescription(invigorating spleen and tonifying kidney)on spleen cell cycle of mice cultured in vitro.[Methods] The influence of Jianpi Bushen Prescription on DNA cycle of mice spleen cell 48 hours after culture was determined with flowcytometry.[Results] The ratio of G0/G1-phase cells in the treatment group with high concentration of the prescription was lower than that in the control group(P<0.01).In addition the ratio of S-phase of cells in all the treatment group at different concentration was higher than that in the control group(P<0.01).But the cells at G2/M-phase was higher only at the high concentration than that in the control(P<0.01).[Conclusion] Jianpi Bushen Prescription may promote the proliferation of the cultured mice spleen cells. |
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