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| Effect of Shen Luo-ning on Extracellular Matrix Metabolism of Kidney Interstitial Tissue in IgA Nephrosis Rats |
| Hits 1650 Download times 1991 Received:December 12, 2005 |
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| DOI
10.11656/j.issn.1672-1519.2006.02.10 |
| Key Words
Shen Luo-Ning;IgA Nephrosis;interstitial;extracellular matrix |
| Author Name | Affiliation | | LIU Yu-jun | The Central Hospital of Shanghai Putuo District, Shanghai 200062, China | | PENG Wen | The Central Hospital of Shanghai Putuo District, Shanghai 200062, China | | WANG Hui-jun | Tianjin University of TCM, Tianjin 300193, China | | 朱小棣 | 天津中医药大学第一附属医院, 天津, 300193 | | 黄文政 | 天津中医药大学第一附属医院, 天津, 300193 |
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| Abstract
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| [Objective] To investigate the effect of Shen luo-ning (calming kidney collateral, SLN) on the extracellular matrix metabolism of kidney interstitial tissue in rats with IgA Nephrosis(IgAN).[Methods] IgAN in rats was established by taking bovine serum albumin(BSA) orally first and then intravenously via tail vein at fixed hour, combined with staphylococcus enterotoxin B (SEB) infection. SLN was given as a treatment. Using in situ hybridization(ISH) immunohistochemistry and computer image analysis the effects of SLN on the expression of transforming growth factor β1 (TGFβ1), collagen type Ⅲ(Col-Ⅲ), inhibitor of plasminogen activator (PAI-1), matrix metalloproteases-1 (MMP-1) and the gene and protein expression of tissue inhibitors of matrix metalloproteases-1 (TIMP-1) in kidney interstitial of IgAN rats were determined.[Results] In kidney interstitial, the mRNA expression of TGFβ1, Col-Ⅲ, PAI-1, TIMP-1 of model group was higher than that of normal group(P<0.01 or P<0.05). In the treatment group, the expression of MMP-1mRNA was higher than model group(P<0.01).[Conclusions] SLN can reduce kidney interstitial pathologic injury in rats woth IgAN . The above-mentioned actions may be related with that SLN can down-regulate the expression of TGFβ1, Col-Ⅲ, PAI-1, TIMP-1 and up-regulate that of MMP-1. This may be the mechanisms of SLN preventing and curing kidney interstitial injury in rats with IgAN. |
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