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| Effects of Liang Jin Mushroom Polysaccharide on IL-2 Generation in Mononuclear Cells of Human Umbilical Blood |
| Hits 1947 Download times 1671 Received:December 20, 2005 |
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| DOI
10.11656/j.issn.1672-1519.2006.02.30 |
| Key Words
LiangJin Mushroom polysaccharide;umbilical blood mononuclear cells;interleukin-2;interleukin-2 mRNA |
| Author Name | Affiliation | | LV Hai-Rong | Tianjin First Central Hospital, Tianjin 300192, China | | LI Yu-Ming | Tianjin First Central Hospital, Tianjin 300192, China | | WANG Jing | Lanzhou Medical College, LanZhou 730000, China |
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| Abstract
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| [Objective] To study the effects of Liangjin mushroom polysaccharide (LJPS) on the secretion of IL-2 and the expression of IL-2 mRNA from the mononuclear cells(CBMC) of human umbilical blood. To explore the immunologic and anti-tumor activity of LJPS and provide the experimental evidence for medical use of LiangJin Mushroom cultivated in China.[Methods] The secretion of IL-2 from CBMC was determined by radioimmunoassay method and the expression of IL-2 mRNA was detected by the reverse transcription polymerase chain reaction (RT-PCR).[Result] Both LJPS alone or combined with PHA could enhance the secretion of IL-2 from CBMC(P<0.05 and P<0.01). The peak was at about 48th hour later. Moreover, they could promote the expression of IL-2 mRNA in CBMC with the peak at about 24th hour.[Conclusion] LJPS can enhance the secretion of the endogenous IL-2 and the expression of IL-2 mRNA of CBMC. |
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