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| Effect of Hui-shen Granule on Apoptosis of Nerve Cells in Ischemic Region After Local Cerebral Ischemia in Rats |
| Hits 1645 Download times 2112 Received:March 08, 2006 |
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| DOI
10.11656/j.issn.1672-1519.2006.03.26 |
| Key Words
Hui-shen granule;cerebral ischemia;apoptosis;Bcl-2 gene;bax gene |
| Author Name | Affiliation | | CHEN Bao-gui | The Hospital of TCM, Wuqing District, Tianjin 300170, China | | ZHAO Zheng-fa | The Hospital of TCM, Wuqing District, Tianjin 300170, China | | BIAN Jing-zhi | The Hospital of TCM, Wuqing District, Tianjin 300170, China | | 李洪艳 | The Hospital of TCM, Wuqing District, Tianjin 300170, China | | 陈慧娲 | The Hospital of TCM, Wuqing District, Tianjin 300170, China | | 赵廷浩 | 天津中医药大学 天津300193 |
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| Abstract
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| [Objective] To observe the effect of Hui-shen(restoring the mental function) capsule on apoptosis, Bcl-2 and Bax protein expression of nerve cells in brain after cerebral ischemia in rats. [Methods] We used Cauterization method to reproduce the MCAO model in Wistar rats and then divided the rats into 3 groups, 10 rats in each group: sham, Chinese herb of Hui-shen granule and control group. Frozen sections of the brain tissue were prepared in immunohistochemistry studies to investigate the expression of Bcl-2 and Bax protein and also in the TUNEL to observe the apoptosis of neural cells. [Results] There was a significant difference between the Hui-shen granule group and the model group with fewer TUNEL, Bax positive cells in the Hui-shen granule group(P<0.01). There was a significant difference between the Hui-shen granule group and the model group with more Bcl-2 positive cells in the Hui-shen granule group as well(P<0.01). there was also a significant difference between the Hui-shen granule group and the model group with higher level Bcl-2/Bax in the Hui-shen granule group(P<0.05). [Conclusions] Hui-shen granule can suppress apoptosis of cerebral ischemia-traumatic nervous cells; Hui-shen granule can enhance the expression of Bcl-2, suppress the expression of Bax and improve the ratio of Bcl-2 to Bax in the brain of rat with MCAO. This may be one of the mechanisms for Hui-shen granule in suppressing apoptosis and protecting the cell of brain. |
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