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| Optimization of process for ultrasonic extraction and primary determination of bacteriostatic activity of protein from flos trollii chinensis |
| Hits 2251 Download times 1728 Received:October 20, 2006 |
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| DOI
10.11656/j.issn.1672-1519.2007.01.30 |
| Key Words
protein from Flos Trollii Chinensis;restrain bacterium action;extraction technical |
| Author Name | Affiliation | | ZHANG Li-juan | | | ZHANG Gui-jun | | | LI Ren-wei | |
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| Abstract
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| [Objective] To optimize the ultrasonic extraction technology for the protein from Flos Trollii Chinensis and determinate the bacteriostatic activity of the protein extract.[Methods] By applThe L9(34) orthogonal test was applied to optimize the ultrasonic extraction technology and the activity of bacteriostasis was determined with slip diffusion method.[Results] The optimized ultrasonic extraction technology was as follows: material granule 60 order,proportion of material to solution 1:30,extracted time 30 min.The extracted protein at 1.25 g/L could inhibit Bacillus proteus(++),Bacillus typhosus(++),Bacillus aerogenes(+),Bacterium coli(+).[Conclusions] The method with ultrasonic extraction can obtain higher plurinatality of protein of Flos Trollii Chinensis without any loss of its biologic activity. |
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