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Influence of Hua Yu Tong Mai injection on proliferation of ECV304 in human umbilical vein
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DOI   10.11656/j.issn.1672-1519.2007.01.35
Key Words   Huayu Tongmai injection;endothelial cell;proliferation
Author NameAffiliation
WANG Jun  
YUAN Zhuo  
TIAN Fei  
张军平  
Abstract
    [Objective] To observe the influence of Hua Yu Tong Mai (Removing stasis and promoting circulation ,HYTM) Injection on the proliferation of ECV304 in human umbilical vein with the serum pharmacological method and provide a clue for choosing target gene for promoting vascular angiogenesis.[Methods] The endothelial cell in logarithmic growth phase was obtained and synchronized in the G0 time.Their proliferation activity was examined by CCK-8; BrdU incorporate was examined with ELISA.[Results] Compared with the blank blood serum control group,the volume increase score of medicine-containing serum with 10% HYTM.[Conclusions] The volume increase score of serum containing medicine of 10% HYTM may promote endothelial cell proliferation through promoting DNA synthesis and mitosis and possibly has protective effect on vascular endothelium.

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