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| Improving of simultaneous determination of periplocin and 4-methoxy salicylic aldehyde in cortex periplocae with HPLC method |
| Hits 2287 Download times 1984 Received:December 12, 2006 |
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| DOI
10.11656/j.issn.1672-1519.2007.03.35 |
| Key Words
cortex periplocae;periplocin;4-methoxysalicylic aldehyde;HPLC |
| Author Name | Affiliation | | REN Xiao-liang | Tianjin University of TCM, Tianjin 300193, China | | LIU Hong | Tianjin University of TCM, Tianjin 300193, China | | QI Ai-di | Tianjin University of TCM, Tianjin 300193, China | | 王焱 | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To establish the method of simultaneous determination of the content of periplocin and 4-methoxysalicylic aldehyde in Cortex periplocae. [Methods] The determination was carried out by HPLC method on ODS chromatographic column, using acetonitrile:methanol:water (27:10:63) as the mobile phase at 220 nm. [Results] The calibration curve was linear within the range of 0.08～1.0 μg, r=0999 4 for periplocin and 0.22～3.0 μg, r=0999 9 for 4-methoxy salicylic aldehyde. The precision of the two constituents was 0.79% and 0.67%, the reproducibility was 1.2% and 2.2% and the recovery rate of samples was 97.1% and 96.2% respectively. [Conclusions] The method can be used to determine periplocin and 4-methoxy salicylic aldehyde simultaneously. It is more convenient and accurate than the method reported in the literatures. |
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