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| Quantitative determination of notoginsenoside R1 and ginsenoside Rg1 in Huoxue Zhitong tablet by RP-HPLC method |
| Hits 1585 Download times 1367 Received:March 10, 2007 |
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| DOI
10.11656/j.issn.1672-1519.2007.05.33 |
| Key Words
Huoxue Zhitong tablet;RP-HPLC;Notoginsenoside R1;Ginsenoside Rg1 |
| Author Name | Affiliation | | CHEN Zai-xing | College of Pharmaceutical Sciences of Chinese Medical University, Shenyang 110001, China | | YUAN Chang-ji | College of Pharmaceutical Sciences of Chinese Medical University, Shenyang 110001, China | | LI Li-hong | College of Pharmaceutical Sciences of Chinese Medical University, Shenyang 110001, China | | 刘丽 | 辽宁中医药大学, 沈阳, 110032 | | 郝艳玲 | 沈阳市骨科医院, 沈阳, 110032 | | 姜泓 | College of Pharmaceutical Sciences of Chinese Medical University, Shenyang 110001, China |
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| Abstract
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| [Objective] To perfect the quality standard of notoginsenoside R1 and ginsenoside Rg1 in the Huoxue Zhitong tablet. [Methods] The chromatographic separation was performed on a Kromasil C18 column with a linear gradient elution of acetonitrile-0.05% phosphoric acid,0~10 min,20% acetonitrile; 10~40 min,20% acetonitrile -30% acetonitrile. Detection wavelength was set at 203 nm; flow rate was 1.0 mL/min and column temperature was set at 30 ℃.[Results] Notoginsenoside R1 and ginsenoside Rg1 had a good linearity in the range of 0.207 2~1.036 μg and 0.8296~4.148 μg,The average recovery of this method was 96.1%,97.3%,RSD=2.02%,2.34%(n=5)respectively.[Conclusions] The procedure is simple and reliable and the results were stable and reproducible. The established method can determine notoginsenoside R1 and ginsenoside Rg1 in the Huoxue Zhitong tablet accurately. |
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