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| Determination of the contents of five kinds of anthraquinones components in Yinxieling Film by HPLC |
| Hits 1610 Download times 1488 Received:February 10, 2007 |
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| DOI
10.11656/j.issn.1672-1519.2007.05.36 |
| Key Words
HPLC;Yinxieling Film;anthraquinones components |
| Author Name | Affiliation | | TONG Jie | TianJin University of TCM, Tianjin 300193, China | | LI Jin | The First Affiliated Hospital to Tianjin University of TCM, Tianjin 300193, China | | CHEN Tao | The First Affiliated Hospital to Tianjin University of TCM, Tianjin 300193, China | | 李凤丽 | TianJin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To establish a method for determining the contents of five kinds of anthraquinones components in Yinxieling(treating psoriasis) Film. [Methods] HPLC was carried out using a irregular-H C18 column (250 mm×4.6 mm 10 μm) and taking methanol-0.1% phosphoric acid(85:15) as the mobile phase with flowing rate at 1.00 mL/min and detecting at 254 nm.[Results] The calibration curve of aloe emodin was linear from 0.784 μg to 15.68 μg (r=0.999 4,n=6) and the average recovery was 100.37 % with RSD 1.31%. The calibration curve of rhein was linear from 0.748 μg to 14.96 μg(r=0.999 6,n=6)and the average recovery was 100.12 % with RSD 1.60 %. The calibration curve of emodin was linear from 0.804 μg to 16.08 μg (r=0.999 8,n=6)and the average recovery was 101.20 % with RSD 1.79 %. The calibration curve of chrysophanol was linear from 0.806 μg to 16.12 μg (r=0.999 4,n=6)and the average recovery was 100.64% with RSD 1.20%. The calibration curve of physcion was linear from 0.46 μg to 9.32 μg(r=0.999 9,n=6)and the average recovery was 101.89% with RSD 0.54%.[Conclusions] This method is proved to be accurate,reliable and simple and can be used for the determination of anthraquinones components in Yinxieling Film. |
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