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| Effect of Xingnao Kaiqiao acupuncture on proteomics in hippocampus in rats with focal cerebral ischemia |
| Hits 2760 Download times 1782 Received:August 31, 2007 |
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| DOI
10.11656/j.issn.1672-1519.2007.06.03 |
| Key Words
Xingnao Kaiqiao(restoring consciousness and waking up the patient from unconsciousness) acupuncture;cerebral ischemia;Proteomics;two-dimensional gel electrophoresis(2-DE);matrix-assisted laser esorption/ionization-time of flight(MALDI-TOF-MS) |
| Author Name | Affiliation | | WEN Jing-Rong | | | ZHAO Xiao-Feng | | | WANG Shu | | | 石学敏 | |
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| Abstract
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| [Objective] To find out the target and effective proteins related with cerebral ischemia and then explore the pathologic mechanism and the acupuncture effect on proteomics in rats with focal cerebral ischemia.[Methods] Wistar rats were randomly divided into normal group, sham operated 6 h group, MCAO 6 h group and acupuncture 6 h group. Nylon monofilament was used to induce middle cerebral artery occlusion (MCAO). After sample preparation, two-dimensional gel electrophoresis was performed. The 2-DE gel was stained with Coomassie brilliant blue R-350. The images were analyzed by software of Image master 2D V3.01 Elite. The peptide mass fingerprints of differentially expressed proteins were detected by MALDT-TOF-MS, then the data base of Swiss-Port to identify the proteins was researched.[Results] There were 30 differentially expressed proteins in normal group, sham operated 6 h group, MCAO 6 h group and acupuncture 6 h group. Compared with normal group, Ubiquinol-cytochrome C reductase iron-sulfur subunit was detected only in MCAO 6 h group; 11 proteins were significantly up-regulated and 17 down-regulated. Compared with acupuncture group, 16 proteins were significantly up-regulated and 5 down-regulated.[Conclusions] From proteomics, we find out a series of proteins related with cerebral ischemia and some parts of target proteins of acupuncture therapy. It can be helpful for deeply researching the ischemic injury mechanism and acupuncture treating mechanism. |
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