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| Effect of acupuncture of Sitian Points on peripheral inflammatory factors in pathologic net in rats with experimental move-static power unbalanced disease of vertebrae cervicales |
| Hits 1566 Download times 1624 Received:October 09, 2007 |
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| DOI
10.11656/j.issn.1672-1519.2007.06.17 |
| Key Words
acupuncture of Sitian Points (ASTP);cervical syndrome;pathologic ring of cervical syndrome;inflammatory factor |
| Author Name | Affiliation | | LIU Gong-wang | | | WANG Xiu-yun | | | ZHOU Zhen | | | 孟向文 | | | 董洪英 | | | 张果忠 | | | 吕少文 | | | 尚秀葵 | | | 王强 | |
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| Abstract
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| [Objective] To study the effect and mechanism of Acupuncture on Sitian Points (ASTP) on the peripheral inflammatory factor in vertebrae cervical syndrome (CS). We induced the imbalance of dynamic and static forces in the cervical disk in Wistar rats, examined the expression of IL-1β, COX-2, iNOS by immunohistochemical method and the expression of IL-1βmRNA, iNOSmRNA by molecular biology method.[Methods] The model of cervical disc dynamic and static forces imbalance was established with the methods used in Shanghai University of TCM, which was similar to the pathogenesis of CS. Sixty Wistar rats were randomly divided into 4 groups with 15 each: SO group, model group, APJJX group, APSTX group. After acupuncture, every group was randomly divided into 3 time groups again with 5 each: the groups of 1, 2, 4 weeks. Then we investigated the effect of APSTX from the followed aspects: to observe the influence on the expression of IL-1β, COX-2, iNOS in intervertebral disc by immunohistochemical SABC method; to observe the influence on the expression of IL-1βmRNA, iNOSmRNA in intervertebral disc by molecular biology RT-PCR method.[Results] APSTP can reduce the expression of COX-2, IL-1β, iNOS and the expression of IL-1βmRNA, iNOSmRNA in the degenerated intervertebral disc of CS model rats through inhibiting the effect of peripheral inflammatory factor.[Conclusions] Puncturing APSTP can markedly delay the process of degenerated intervertebral disc in CS rats. The mechanism of action may be correlated with reducing the effect of peripheral inflammatory factor. |
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