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| Effects of different acupuncture methods on P15 expression of astrocyte in different encephalic regions |
| Hits 1551 Download times 1678 Received:September 12, 2007 |
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| DOI
10.11656/j.issn.1672-1519.2007.06.28 |
| Key Words
"XNKQ" acupuncture method;Alzheimer’s disease(AD);cell cycle factor(P15);encephalic region;SAMP8 |
| Author Name | Affiliation | | FAN Xiao-nong | | | LIU Jian | | | LI Tan | | | 张岭 | | | 王舒 | |
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| Abstract
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| [Objective] To understand the expression regulation of cell cycle related factor P15(cyclin-dependent kinase inhibitor)of astrocyte in different encephalic regions in AD model mouse(SAMP8), and the effects of different acupuncture prescriptions(XNKQ prescription and reinforcing deficiency prescription) on it.[Methods] Astrocyte(GFAP) and P15 in SAMP8 brain were respectively labeled with immunohistochemistry double stain technique. By using pathologic image analysis technique, the positive cells both labeled wi GFAP and P15 were observed from four encephalic regions: cortex, hippocamp, striatum and bulbus cinereus.[Results] Compared with normal group, the quantity of positive cells reduced in hippocampus of AD model group. There was no statistically significant changes among other 3 encephalic regions. "XNKQ" group could raise the expression of positive cells in hippocamp and bulbus cinereus, and reinforcing deficiency group could only raise that of in hippocamp (P<0.05).[Conclusions] It may be an important pathogenesis of AD that the expression of P15 in astrocytes of hippocamp in 8-month age SAMP8 reduces. Acupuncture can promote the expression of P15 in astrocytes locating at different encephalic regions, and the target area is different for different acupuncture prescriptions. It can be potential mechanism of "XNKQ" acupuncture method on AD that P15 expression of astrocytes increases in hippocamp and bulbus cinereus. |
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