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| Effect of Jinsiwei on neuron microtubule in hippocampus and expression of Tau-protein phosphorylation related enzyme in rats with AD |
| Hits 1755 Download times 1898 Received:August 30, 2007 |
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| DOI
10.11656/j.issn.1672-1519.2008.01.27 |
| Key Words
Alzheimer’s disease;Tau-protein;microtubule;GETO |
| Author Name | Affiliation | | CHEN Yu-jing | 北京中医药大学东直门医院老年病科, 北京, 100700 | | WANG Rong | 教育部神经变性病学重点实验室, 北京, 100054 | | SHENG Shu-li | 教育部神经变性病学重点实验室, 北京, 100054 | | 姬志娟 | 教育部神经变性病学重点实验室, 北京, 100054 | | 赵志炜 | 教育部神经变性病学重点实验室, 北京, 100054 | | 尹军祥 | 北京中医药大学东直门医院老年病科, 北京, 100700 | | 时晶 | 教育部神经变性病学重点实验室, 北京, 100054 | | 田金洲 | 教育部神经变性病学重点实验室, 北京, 100054 |
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| Abstract
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| [Objective] To study the Jinsiwei(GETO)-induced changes in the ultrastructure of neuron microtubule in CAI region of hippocampus and the expression of enzymes related to phosphorylation of tau-proteins in AD rats. [Methods] Forty-eight SD rats were randomly divided into sham operation group, model group, donepezil group, the high, middle and low dosage of GETO group. AD was induced by intracerebroventricular administration of streptozotocin (ic-STZ). The GETO groups were treated with gastric perfusion of Jinsiwei in three different dosage for three months. Both the model group and sham group were given double distilled water. Three months later, intravascular fixative was injected into the rats. Tissue specimens for each group were harvested from the hippocampus CA1 area for light and electron microscopy studies. Cryostatsecions were studied with immunohistochemistry for glycogen synthase kinase 3β(GSK-3β), protein phosphatase-1 (PP-1), protein phosphatase-2A (PP-2A). [Results] The microtubules of hippocampus neurons of AD showed prominent changes of fragmentation and dissolution, while treatment with GETO can significantly ameliorate these changes. The hippocampus neurons of AD rats showed significantly intracellular increase in the tau-phosphokinase, GSK-3β and decrease in the tau-dephosphrylating enzymes, PP-1 and PP-2A. GETO can also restore the expression of enzymes to normal levels,and there was no difference between the Donepezil group and GETO groups. [Conclusions] Brain tissue of AD rats shows changes in microtubules and enzymes related to phosphorylation of tau proteins. GETO can improve all the abnormalities of AD rats. |
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