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| Effect of acupuncture and construction of differential expression map of hippocamp proteome in rats with focal cerebral ischemia |
| Hits 1785 Download times 1571 Received:September 20, 2007 |
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| DOI
10.11656/j.issn.1672-1519.2008.02.04 |
| Key Words
acupuncture;cerebral ischemia;proteome;two-dimensional electrophoresis;MALDI-TOF-MS |
| Author Name | Affiliation | | WEN Jing-rong | Department of Acupuncture of Tianjin University of TCM, Tianjin 300193, China | | ZHAO Xiao-feng | The First Hospital Affiliated to Tianjin University of TCM, Tianjin 300193, China | | WANG Shu | The First Hospital Affiliated to Tianjin University of TCM, Tianjin 300193, China | | 石学敏 | The First Hospital Affiliated to Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To construct differential expression map of hippocamp proteome in rats with focal cerebral ischemia to search the related protein of cerebral ischemia and explore the pathogenesis mechanism of cerebral ischemia and the effect of acupuncture.[Methods] Wistar rats were randomly divided into normal group,sham operation group,model group,Xingnao Kaiqiao(restoring consciousness and waking up the patient from unconsciousness) acupuncture group and traditional acupuncture control group.Focal cerebral ischemia was induced by line-bolted method in rats.Image analysis by Image Master 2D V3.01 Elite software was carried out and the finger print map of peptide quality by MALDI-TOF-MS method was measured.The Swiss-Port database was retrieved and protein appraisement was carried out.[Results] There wasn’t differential protein expression in normal group and sham operation group.Compared with the normal group twenty-nine differential protein particles was identified in model group.The differential protein particles included one particle emerged in 6 h group,eleven particles with upregulating expression and seventeen particles with downregulating.Compared with model group twenty-one differential protein particles in Xingzhen group were identified including sixteen particles with upregulating expressing and five particles with downregulating.Compared with model group twenty differential protein particles in acupuncture control group were identified including twelve particles with upregulating expression and eight particles with downregulating.[Conclusions] The partial related protein with cerebral ischemia was discovered by two-dimensional electrophoresis associated with mass spectra technique.The study result is helpful for lucubrating the pathogenesis mechanism of cerebral ischemia injury and discovering the effect mechanism of partial target protein in treating cerebral ischemia. |
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