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| Determination of LDH-X activity in testes of mice with deficiency of Kidney-yang induced by overstrain and intemperate Sexual Intercourse |
| Hits 1783 Download times 1951 Received:November 17, 2007 |
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| DOI
10.11656/j.issn.1672-1519.2008.02.24 |
| Key Words
deficiency of kidney-yang;mice;LDH;LDH-X |
| Author Name | Affiliation | | LI Zhen | The College of Basic Medicine, Shandong University of TCM, Jinan 250014, China | | LIU Li-qing | The College of Basic Medicine, Shandong University of TCM, Jinan 250014, China | | YAN Ting-xiang | Shandong University of TCM, Jinan 250014, China | | 李淑燕 | The College of Basic Medicine, Shandong University of TCM, Jinan 250014, China | | 艾邸 | The College of Basic Medicine, Shandong University of TCM, Jinan 250014, China | | 宋辉 | Shandong University of TCM, Jinan 250014, China | | 闫川慧 | Shandong University of TCM, Jinan 250014, China | | 王媛 | Shandong University of TCM, Jinan 250014, China | | 慕小婧 | Shandong University of TCM, Jinan 250014, China | | 刘鹏 | Shandong University of TCM, Jinan 250014, China | | 郭根艳 | Shandong University of TCM, Jinan 250014, China |
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| Abstract
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| [Objective] The changes of LDH and LDH-X activity in testes of mice with deficiency of kidney-yang induced by overstrain and intemperate sexual intercourse were examined.[Methods] The contents of pyruvate in testis homogenates was measured and the total LDH activity and LDH-X isoenzyme activity per gram of wet tissue were determined by 2,4-dinitrophenylhydrazine color test in different groups.[Results] The total LDH activity in model group of deficiency of kidney-yang was significantly lower than that in normal control group(P<0.05,P<0.01 respectively)while it was higher in therapy group with Jingui Shenqi pill P<0.05,P<0.01)compared with that in model group.LDH-X activity in the second week in model group was decreased compared with that in control group,but there is no statistical difference.Until the fourth and sixth week the decrease became significant(P<0.05);Compared with LDH-X activity in model group,it was raised in the second week in therapy group,but statistics showed no significance.But it was increased significantly in the fourth and sixth week(P<0.01).[Conclusions] The ground of modern science of "the kidney dominating reproduction" is proved by the changes of LDH-X(specific enzyme of sperm) activity and this model is the one with deficiency of kidney-yang. |
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