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Effects of Zishen Tiaochong method on ovaries VEGF expression in rats with induced ovulation
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DOI   10.11656/j.issn.1672-1519.2008.02.27
Key Words   Zishen Tiaochong Method;vascular endothelial growth factor;immunohistochemical method;experimental study
Author NameAffiliation
LI Rui-huan Tianjin University of TCM, Tianjin 300193, China 
WANG Ling Tianjin Hospital of Orthopedics, Tianjin 300211, China 
SUN Yan-ming Tianjin Hospital of Orthopedics, Tianjin 300211, China 
徐瑾 天津中医院 天津 300211 
陈静 Tianjin Hospital of Orthopedics, Tianjin 300211, China 
张远 Tianjin Hospital of Orthopedics, Tianjin 300211, China 
Abstract
    [Objective] To observe the effects of Zishen Tiaochong method on ovaries VEGF expression of rats with induced ovulation.[Methods] Eighty rats were randomly divided into four groups: the high-dose and low-dose of GongxueningⅡgroup,positive control group and model group.The VEGF expression at ovary follicular development stage and preovulatory stage of four groups was detected with immunohistochemistry.[Results] During follicular development stage and preovulatory stage,the area,MOD,area%,IOD of GongxueningⅡ group were obviously higher than that of model group.The VEGF expression of preovulatory stage was all higher than that of follicular development stage in four groups.[Conclusions] The molecular mechanism of Zishen Tiaochong Method is possibly concerned with the promotion on ovarial VEGF expression and improvement of follicular blood supply.

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