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| Effect of Zishui Qinggan Decoction on hypothalamus ER-α mRNA expression and 5-HT content in rats with Depression in peri-menopausal period |
| Hits 1708 Download times 1919 Received:November 08, 2007 |
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| DOI
10.11656/j.issn.1672-1519.2008.02.39 |
| Key Words
depression model in peri-menopausal period;Zishui Qinggan decoction;ER-α mRNA;5-HT |
| Author Name | Affiliation | | JIANG Da-zhen | Korea | | FENG Qiu-xia | Tianjin Third Central Hospital, Tianjin 300170, China | | GAO Ying-tang | The First Hospital Affiliated to Tianjin University of TCM, Tianjin 300193, China | | 金季玲 | 天津中医药大学第一附属医院 天津 300193 |
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| Abstract
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| [Objective] To observe the effect of Zishui Qinggan decoction on hypothalamus ER-α mRNA expression and 5-HT content in rats with depression in peri-menopausal period.[Methods] Forty-five rats were randomly divided into five groups: control group,model group,TCM group,Estradiolum group and Estradiolum and Fluoxetine group.The rats of control group were treated with sham operation.The rats of other groups were treated with ovariosteresis and chronicity non-prediction stress.The rats of control and model group were administrated with physiologic saline and that of other group were individually administrated with Zishui Qinggan decoction,Estradiolum,Estradiolum and Fluoxetine.Hypothalamus ER-α mRNA expression and 5-HT content were determined by RT-PCR and HPLC-electrochemical detector method.[Results] The ER-α mRNA expression of model group was obviously lower than that of control group(P<0.01).The ER-α mRNA expression of TCM group was obviously higher than that of model group(P<0.05).The 5-HT of model group was obviously lower than that of control group(P<0.01) and The 5-HT of TCM group was obviously higher than that of control group(P>0.05).[Conclusions] The ER-α mRNA expression and 5-HT content exhibits low expression in rats of model group.Zishui Qinggan decoction may increase the content of 5-HT and increase the expression of ER-α mRNA and improve the condition of rats with depression in peri-menopausal period by enhancing ER-α mRNA transcriptional level and biological effect. |
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