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| Simultaneous determination the content of tetrahydropalmatine and protopine in corydalis decumbens (Thunb.) Pers. simultanelusly with RP-HPLC |
| Hits 1791 Download times 2030 Received:December 01, 2007 |
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| DOI
10.11656/j.issn.1672-1519.2008.04.28 |
| Key Words
Corydalis decumbens (Thunb.) Pers.;tetrahydropalmatine;protopine;RP-HPLC |
| Author Name | Affiliation | | Chen zai-xing | School of Pharmaceutical Sciences, Chinese Medical University, shenyang 110001, China | | Meng shu | Liaoning Institute of Family Planning, shenyang 110031, China | | Jiang hong | Chinese Medical university, shenyang 110001, China | | 李丽红 | School of Pharmaceutical Sciences, Chinese Medical University, shenyang 110001, China | | 孟繁浩 | School of Pharmaceutical Sciences, Chinese Medical University, shenyang 110001, China |
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| Abstract
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| [Objective] A RP-HPLC method is established for simultaneous determination of the content of protopine and tetrahydropalmatine in Corydalis decumbens (Thunb.) Pers from the different habitats. [Methods] The chromatographic separation was performed on a Kromasil C18 column with a linear gradient elution of acetonitrile-0.1% phosphoric acid was used. The detection wavelength was set at 280 nm; flow rate was 1.0 mL/min and column temperature was set at 35 ℃. [Result] Protopine and tetrahydropalmatine had a good linearity in the range of 0.22～1.10 μg and 0.174～0.870 μg,The average recoveries of this method were 98.9% and 99.71%;RSD were 1.6% and 1.8%(n=5)respectively and detected at wavelength of 280 nm. [Conclusion] The contents of the two alkaloids in Corydalis decumbens (Thunb.) Pers. from the different habitats show obvious difference. The method is simple,accurate,quick and suitable to determine content and evaluate quality for the herds. |
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