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| Inducing effect of super-antigen SEA on human peripheral blood monocytes in killing breast cancer cells |
| Hits 1779 Download times 1843 Received:May 11, 2008 |
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| DOI
10.11656/j.issn.1672-1519.2008.05.23 |
| Key Words
staphylococcalenterotoxin A(SEA);peripheral blood mononuelear cell(PBMC);anti-tumor effect |
| Author Name | Affiliation | | ZHU Xiu-shan | The Bioengineering College of Tianjin University of Science and Technology, Tianjin 300457, China | | SUN Jia-lin | The Bioengineering College of Tianjin University of Science and Technology, Tianjin 300457, China | | HU Kun | The Bioengineering College of Tianjin University of Science and Technology, Tianjin 300457, China | | 张世奇 | The Bioengineering College of Tianjin University of Science and Technology, Tianjin 300457, China | | 王雪 | The Bioengineering College of Tianjin University of Science and Technology, Tianjin 300457, China | | 冯艳敏 | The Bioengineering College of Tianjin University of Science and Technology, Tianjin 300457, China |
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| Abstract
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| [Objective] To study the enhancing effect of super-antigen SEA on human peripheral blood monocytes (PBMC) in killing breast cancer cells (MCF-7). [Methods] PBMC was separated by gradient centrifugation from human peripheral blood, then different concentrations of super-antigen SEA, 0.01, 0.1, 1,10mg/L were added and Co-incubated with MCF-7 in plate of 96 wells for 24,48 and 72 h. Then the cytotoxicity on tumor was detected. [Results] With the increase of time, the cytotoxicity on tumor induced by SEA was enhanced. The tumor-cytotoxicity effect was the strongest when stimulated PBMC with super-antigen SEA of 10 kg/L at 72 h later.[Conclusion] Super-antigen SEA can enhance the anti-cancer effect of human peripheral blood mononuclear cells (PBMC). |
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