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| Comparative study on effect of shenfu injection and shengmai injection on Ca2+-ATPase of myocardial cell in Mice |
| Hits 1482 Download times 1935 Received:April 14, 2008 |
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| DOI
10.11656/j.issn.1672-1519.2008.06.22 |
| Key Words
Shenfu injection;Shengmai injection;myocardial cells;Ca2+-ATPase |
| Author Name | Affiliation | | LUO Ming-feng | Chengdu University of TCM, Chengdu 610075, China | | ZHANG San-yin | Chengdu University of TCM, Chengdu 610075, China | | HUANG Xiu-shen | Chengdu University of TCM, Chengdu 610075, China | | 周艳霞 | Chengdu University of TCM, Chengdu 610075, China | | 孙改侠 | Chengdu University of TCM, Chengdu 610075, China |
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| Abstract
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| [Objective] To explore the effect of Shenfu injection and Shengmai injection on Ca2+-ATPase of myocardial cell in mice and provide the basis for the clinical application. [Methods] Using trypsin digestion the myocardial cells were cultured in vitro. After purifying the cells with adhering to the wall with different velocity Shenfu injection and Shengmai injection with three concentrations gradient were added directly to the myocardial cells in logarithmic developing period. Two hours later its effects on the activity of Ca2+-ATPase in myocardial cells was detected. [Results] Shenfu injection of the low, middle and high concentration could increase the Ca2+-ATPase activity of myocardial cells, and the effect of middle concentration was the most obvious. (P<0.05) Lower effect of low and middle concen-tration of Shengmai Injection was found. The high concentrations of Shenfu Injection, however, developed a increasing trend of the Ca2+-ATPase activity. [Conclusion] It is indicated that the effect of Shenfu injection on the heart may be related to the increasing of Ca2+-AT-Pase activity in myocardial cells;Shengmai injection has some effect on Ca2+-ATP activity in myocardial cells to a certain extent through other mechanisms on the myocardial cells. |
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