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| In vivo and in vitro research on anti-tumor effects of Gekko Polysaccharide on murine H22 hepatoma |
| Hits 1663 Download times 1791 Received:April 28, 2008 |
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| DOI
10.11656/j.issn.1672-1519.2008.06.26 |
| Key Words
Gekko polysaccharide;anti-tumor activity |
| Author Name | Affiliation | | YANG Li-hua | Department of Integrated Chinese Medicine and Western Medicine, The First Hospital of Peking University, Beijing 100043, China | | YANG Jin-xia | Department of Integrated Chinese Medicine and Western Medicine, The First Hospital of Peking University, Beijing 100043, China | | WANG Xue-mei | Department of Integrated Chinese Medicine and Western Medicine, The First Hospital of Peking University, Beijing 100043, China | | 富宏 | Department of Integrated Chinese Medicine and Western Medicine, The First Hospital of Peking University, Beijing 100043, China | | 刘庚信 | Department of Integrated Chinese Medicine and Western Medicine, The First Hospital of Peking University, Beijing 100043, China |
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| Abstract
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| [Objective] To investigate the in vitro inhibitory effect of Gekko polysaccharide on the proliferation of cultured H22 cell of liver cancer in mice. [Methods] MTT assay was applied to evaluate the killing effect of Gekko polysaccharide on cultured H22 hepatoma cells in vitro. The anti-tumor effect of Gekko polysaccharide was observed in H22 tumor bearing mice. [Results] The results showed each dose of Gekko polysaccharide group had effective anti -proliferation effect on the H22 cells in vitro. The highest inhibition ratio was 79.1%,far more higher than 5-FU group, in which inhibition ratio was 48.7%. This result was consistent with Wu. The inhibitory rate of high dose of Gekko polysaccharide, however, was only 8.8% in vivo. There was no difference when compared with the control group (P>0.05). The influence to the thymus, thymus index, spleen and spleen index of mice with transplanted H22 tumors in each Gekko polysac-charide groups were not different with those in the control group (P>0.05). In the 5-FU group these indexes were obviously depressed when compared with those in the control group and Gekko polysaccharide groups(P<0.05). [Conclusion] Gekko polysaccharide has good anti-tumor effect in vitro. However, the inhibition rate of Gekko polysaccharide was low in vivo. Experiments in vitro and in vivo should be combined to make experimental results much more persuasive. Whether Gekko polysaccharide of gecko was the anti-tumor active in-gredients remains further explored. |
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