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| Preliminary study on apoptosis in HepG-2 of human hepatoma cell line induced by CSS |
| Hits 1589 Download times 1821 Received:August 11, 2008 |
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| DOI
10.11656/j.issn.1672-1519.2008.06.32 |
| Key Words
CSS;Human Heptocarcinoma Cell Line HepG-2;Apoptosis;cell cycle change |
| Author Name | Affiliation | | Yu Lei | The Chinese Pharmacy College of Beijing University of TCM, Beijing 100102, China
Harbin University of Commerce, Harbin 150076, China
Engineering Research Center of Natural Anti-cancer Drugs, Ministry of Education, Harbin 150076, China | | Cui Rong-tian | Harbin University of Commerce, Harbin 150076, China
Engineering Research Center of Natural Anti-cancer Drugs, Ministry of Education, Harbin 150076, China | | Mo Ke | Harbin University of Commerce, Harbin 150076, China
Engineering Research Center of Natural Anti-cancer Drugs, Ministry of Education, Harbin 150076, China | | 王崴 | Harbin University of Commerce, Harbin 150076, China
Engineering Research Center of Natural Anti-cancer Drugs, Ministry of Education, Harbin 150076, China | | 季宇彬 | The Chinese Pharmacy College of Beijing University of TCM, Beijing 100102, China
Harbin University of Commerce, Harbin 150076, China
Engineering Research Center of Natural Anti-cancer Drugs, Ministry of Education, Harbin 150076, China | | 邹翔 | The Chinese Pharmacy College of Beijing University of TCM, Beijing 100102, China
Harbin University of Commerce, Harbin 150076, China
Engineering Research Center of Natural Anti-cancer Drugs, Ministry of Education, Harbin 150076, China |
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| Abstract
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| [Objective] To discuss the killing and apoptosis-inducing effect of total alkaloid in the CSS (Capparis spinosa L. saponin, CSS) on human heptocarcinoma cell Line HepG-2. [Methods] The killing effect of the CSS on cell Line HepG-2 was observed by MTT method. Morphological observation of the HepG-2 cells was completed by fluorescence microscope. This test was signed to observe the changes of the cell cycle of HepG-2 affected by the CSS with PI single-staining. [Results] CSS had inhibiting effect on the proliferation of HepG-2 and its IC50 value was 46.16 μg/mL. Characteristic apoptosis appeared after the HepG-2 cells was affected with CSS, The cell cycle changed obviously that the progresses of cells cycle of G1 period and G2 period in high dosage group have been blocked, and the cellular proportion in G2 period is decreased by the action of CSS for 24h. The apoptosis percentage is increased to 66.652% in the 50μg/mL dosage group. [Conclusion] The CSS has obvious killing and apoptosis-inducing effect on human heptocarcinoma cell Line HepG-2 and the cell cycle changes have been observed. |
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