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| Determination of vanillic acid and cinnamic acid in picrohiza scrophulariiflora pennell with HPLC |
| Hits 2264 Download times 2053 Received:June 03, 2008 |
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| DOI
10.11656/j.issn.1672-1519.2008.06.33 |
| Key Words
Picrohiza Scrophulariiflora Pennell;HPLC;Vonillic acid;cinnamic acid |
| Author Name | Affiliation | | YAN Hai-hong | Department of Pharmacy of Blood Disease Hospital of Chinese Academy of medical Sciences, Tianjin 300020, China | | LI Bao-sheng | Department of Pharmacy of Blood Disease Hospital of Chinese Academy of medical Sciences, Tianjin 300020, China | | ZHANG Ya-qian | Department of Pharmacy of Blood Disease Hospital of Chinese Academy of medical Sciences, Tianjin 300020, China |
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| Abstract
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| [Objective] To establish HPLC method for the determination of Vonillic acid and cinnamic acid in rhizoma and fibre of picro-hiza Scrophulariiflora Pennell.[Methods] The column was TianHe C18 (150 mm×4.6 mm) with 10 μm packing. The mobile phase used was methanol-phosphorus acid(40:60). The flow rate was 1.0mL·min-1, detection Wavelength was 260 nm. [Results] The linearity of this method was good in the ranges of 0.72-72 μg for Vonillic acid (r=0.999 8);0.35-3.50 μg for cinnamic acid (r=0.9999). The recoveries of Vonillic acid and cinnamic acid were 99.62% and 98.84%. RSD were 0.75% and 1.65% respectively. [Conclusion] The method is accu-rate, precise and suitable to determine Vonillic acid and cinnamic acid in Picrohiza Scrophulariiflora Pennell. |
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