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| Influence of radix dipsaci containing serum on proliferation of osteoblasts in rats with different sex |
| Hits 1689 Download times 2078 Received:September 12, 2008 |
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| DOI
10.11656/j.issn.1672-1519.2009.01.04 |
| Key Words
osteohlasts;radix despair containing serum;radix dipsaci |
| Author Name | Affiliation | | WANG Wei | Tianjin University of TCM, Tianjin 300193, China | | LIU You-gang | Tianjin University of TCM, Tianjin 300193, China | | SHI Hong | Tianjin University of TCM, Tianjin 300193, China | | 刘友刚 | Tianjin University of TCM, Tianjin 300193, China | | 王超 | Tianjin University of TCM, Tianjin 300193, China | | 郑纺 | Tianjin University of TCM, Tianjin 300193, China | | 杨琳 | Tianjin University of TCM, Tianjin 300193, China | | 顾志敏 | Tianjin University of TCM, Tianjin 300193, China | | 鲍丽颖 | Tianjin University of TCM, Tianjin 300193, China | | 胡利民 | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To observe the effect of Radix Dipsaci containing serum on the proliferation of osteoblasts in rats withdiscontinued administration and different sex.[Methods] The drug containing serum in rats was prepared.Twenty-four Wistar rats withequal male and female were randomly divided into four groups:male control group (Ⅰ), male drug-added group (Ⅱ), female control group (Ⅲ), female drug-added group (Ⅳ), Intragastric perfusion with Radix Dipsaci (4.5g crude drug/kg body weight) and saline respectivelyaccording to the animal body weight was given for one week and then the blood was gained.After centrifugation the supernatant fluid wasobtained.Then the osteoblasts were isolated and cultured.The proliferation of osteoblasts was analyzed quantitatively.The cells weredisposed and added with different concentration of rat serum, and divided into 2.5%, 5% and 10% Radix Dipsaci group.The proliferationof cells was tested with MTT.[Results] When the medium concentration was 2.5% of Radix Dipsaci significant difference was foundbetween male and female drug-added group (P<0.05).There was also significant difference between female drug-added and femaleantitheses group when the medium was 5% concentration of Radix Dipsaci rat serum (P<0.01).While the medium was 10% concentrationof Radix Dipsaci significant difference could be seen between female drug-added group and female antitheses group (P<0.05).However,no matter the concentration of Radix Dipsaci was, no significant differences could be seen between male drug-added group and malecontrol group.[Conclusion] Radix Dipsaci can stimulate the proliferation of osteoblasts.This function in female drug-added group wasmore significant than that of male drug added group. |
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