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| Effect of TongluoJiunao injection on MIP-1β secretion of cerebral micro vascular endothelial cells in rats with oxygen-glucose deprivation |
| Hits 1826 Download times 1929 Received:November 11, 2008 |
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| DOI
10.11656/j.issn.1672-1519.2009.01.05 |
| Key Words
cerebral microvascular endothelial cells;oxygen-glucose deprivation;MIP-1β;Tongluo formula |
| Author Name | Affiliation | | WANG Jun | The Preclinical Medicine College of Beijing University of TCM, Beijing 100029, China | | LI Peng-tao | The Preclinical Medicine College of Beijing University of TCM, Beijing 100029, China | | HOU Jin-cai | The Preclinical Medicine College of Beijing University of TCM, Beijing 100029, China | | 华茜 | The Preclinical Medicine College of Beijing University of TCM, Beijing 100029, China | | 潘彦舒 | The Preclinical Medicine College of Beijing University of TCM, Beijing 100029, China | | 陈会丛 | The Preclinical Medicine College of Beijing University of TCM, Beijing 100029, China | | 杜欢 | The Preclinical Medicine College of Beijing University of TCM, Beijing 100029, China |
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| Abstract
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| [Objective] We addressed our study on the effect of Tongluo Jiunao injection (TLJN) on the MIP-1β secretion of cerebral mi-cro vascular endothelial cells and chemokine receptor 5 (CCR5) under the condition of oxygen-glucose deprivation in rats.[Methods] The oxygen-glucose deprivation was set up, and defined the third passage endothelial cells as four groups:normal endothelial cells (N),normal endothelial cells with TLJN (NT), oxygen-glucose deprivation cells (I), oxygen-glucose deprivation cells with TLJN (IT).The MIP-1βcontent of four supernatants was detected by ELISA and the MIP-1β and it’s receptor CCR5 expression were observed by immunohisto-chemistry and Western blot.[Results] Endothelial cells after deprivation of oxygen-glucose could secrete a lots of MIP-1β into the super-natant with increased expression of MIP-1β and CCCR5 in the injured cells.The content of MIP-1β in the medium of IT was significantlyreduced (P<0.01), the expression of MIP-1β and CCR5 was also decreased in different extent accordingly.[Conclusion] TLJN can signif-icantly inhibit the secretion of MIP-1β into the supernatant and the expression of MIP-1β and CCR5 when micro vascular endothelialcells was injured by oxygen-glucose deprivation.It was presumed that the TLJN’s effect of inhibiting the release of chemokine may be thepotential way of its pharmacodynamic action. |
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