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| Effects of EPCs preconditioned with salvianolic acid B combined with BMSC transplantation into infracted myocardium on expression of cardiac muscle VEGF and bFGF protein in rats |
| Hits 1568 Download times 2104 Received:November 11, 2008 |
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| DOI
10.11656/j.issn.1672-1519.2009.01.27 |
| Key Words
salvianolic acid B;endothelial progenitor cells;acute myocardial infarction;bone mesenchymal stem cells;cellular transplantation;VEGF;bFGF |
| Author Name | Affiliation | | LI Qing-Wen | Dept. of Health &
Exercise Science, Tianjin Institute of Physical Education, Tianjin 300381, China
Tianjin Univercity of TCM, Tianjin 300193, China | | TAN Jun-Zhen | Tianjin Univercity of TCM, Tianjin 300193, China | | NAN Ya-Yun | Tianjin Univercity of TCM, Tianjin 300193, China | | 范英昌 | Tianjin Univercity of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To investigate the effect of Salvianolic acid B-preconditioned endothelial progenitor cells (EPCs) combined withbone mesenchymal stem cells (BMSCs) on angiogenesis in rats with acute myocardial infarction (AMI).[Methods] EPCs and BMSc werecultivated in vitro, purified by density-gradient centrifugation and plastic adherence method and identified by immunofluorescence andimmunocytochemistry (CD34/CD133/CD44).Acute myocardial infarction (AMI) was produced in rats by ligation of the coronary artery.EPCs preconditioned with Salvianolic acid B combined with BMSCs was injected into the five points of heart ischemic area.Four weekslater, we examined rat by ultrasonocardiograph, and the expression of Nkx2.5, GATA-4 by real-time PCR.[Results] The IOD of VEGFand bFGF in EPCs and BMSCs group were 13.179±3.053, 23.634±4.705 respectively.It was increased remarkably comparing with thecontrol group.[Conclusion] The EPCs preconditioned with Salvianolic acid B can improve the vascular microenvironment of BMSCstransplantation. |
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