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| Effect of SalB on expression of desmin and a-actin mRNA in differentiation process of bone marrow mesenchymal stem cells into cardiomyocytes in rats |
| Hits 1799 Download times 1858 Received:September 12, 2008 |
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| DOI
10.11656/j.issn.1672-1519.2009.02.27 |
| Key Words
Salvianolic acid B;bone marrow mesenchymal stem cells;5-azacytidine;Desmine;a-actin |
| Author Name | Affiliation | | HUA Sheng-yu | Tianjin University of TCM, Tianjin 300193, China | | FAN Ying-chang | Tianjin University of TCM, Tianjin 300193, China | | MA Yi-wen | Tianjin University of TCM, Tianjin 300193, China | | 王强 | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To study the differentiation of bone marrow mesenchymal stem cells (MSCs) into cardiomyocytes induced by 5-azacytidine and SalB in vitro and the expressions of Desmin and a-actin to decide the requirements,rules and characteristics of MSCs differentiated into cardiomyocytes. [Methods] The density gradient centrifugation and adherent filtration were used to isolate and purify MSCs. The MSCs were cultured and amplified. MSCs surface antigen profiles were obtained by immunity tissue chemistry. The MSCs of the ninth passage were induced with 5-azacytidine at 10 μmol /L and SalB at 250 mg/L for 24 hours,and the expressions of Desmin and a-actin were tested by RT-PCR at the 4th week,respectively after treating MSCs with 5-azacytidine and SalB. [Results] The samples of MSCs of ninth passage were tested repetitively. The MSCs were positive for CD44 and negative for CD34. After the MSCs treated with 5-azacytidine and SalB for 24 hours,their morphology and arrangement changed obviously. Four weeks later,the cells became small,and the multinuclear myotube-shape constructions were formed by a few cells. The Desmin and a-actin were positive in the MSCs treated group,and negative in control group. With the culture time passed,the levels of Desmin and a-actin in MSCs increased. [Conclusion] MSCs can be induced into cardiomyocytes with 5-azacytindine and SalB can promote it. |
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