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| Determination of chlorogenic acid and establishment of fingerprint spectrum of cortex dictamni produced in Hebei Province |
| Hits 1860 Download times 1733 Received:December 25, 2008 |
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| DOI
10.11656/j.issn.1672-1519.2009.02.28 |
| Key Words
Cortex Dictamni;chlorogenic acid;HPLC;fingerprint |
| Author Name | Affiliation | | LIU Jun-hong | Institute of Acute Abdomen of Traditional Chinese Medicine and Western Medicine, Tianjin 300100, China | | LI Di-hua | Institute of Acute Abdomen of Traditional Chinese Medicine and Western Medicine, Tianjin 300100, China | | WU Xiao-xian | Institute of Acute Abdomen of Traditional Chinese Medicine and Western Medicine, Tianjin 300100, China |
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| Abstract
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| [Objective] To study the determination of chlorogenic acid and establishment of fingerprint spectrum of Cortex Dictamni produced in Hebei province by reversed phased high performance liquid chromatography. [Methods] A Kromasil C18 column was used;the mobile phase was composed of acetonitrile-water with gradient elution. The flow rate was 10 mL/min;The temperature of column was 35℃ and the wavelength of detection was 327 nm. [Results] The mutual mode of HPLC fingerprint was set up,and comparison of similarity was carried out. [Conclusion] Under the selected chromatographic conditions,Chlorogenic acid of Cortex Dictamni and other composi-tion was separated well,and the Quality of Cortex Dictamni can be controlled effectively by HPLC fingerprint. |
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