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| Determination of gallic acid and loganin in fructus corni with HPLC switching UV wavelength |
| Hits 1365 Download times 1504 Received:December 23, 2008 |
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| DOI
10.11656/j.issn.1672-1519.2009.02.29 |
| Key Words
switching UV wavelengths;Fructus Corni;Gallic acid;Loganin |
| Author Name | Affiliation | | LIU Ya-nan | Tianjin University of TCM, Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin 300193, China | | GUO Jin-ming | Tianjin University of TCM, Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin 300193, China | | WANG Yue-fei | 天津中医药大学, 省部共建教育部方荆学重点实验室, 天津, 300193 | | 戚爱棣 | 天津中医药大学, 省部共建教育部方荆学重点实验室, 天津, 300193 |
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| Abstract
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| [Objective] To establish the method for Simultaneous determination of Gallic acid and Loganin in Fructus Corni with switching UV wavelength. [Methods] The HPLC analysis was performed on hypersil C18 column by using gradient elution. The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution;detection wavelength 0-6 min:270 nm,6.01-25 min:240 nm;the column temperature was maintained at 25 ℃;the flow rate was 1 mL/min. [Results] Gallic acid,Loganin showed good linearity in the correspondent concentration;the mean recovery was 103.6% with RSD=0.36%,103.8% with RSD=0.65%. [Conclusion] The method was accurate,repeatable and simple for analysis of Fructus Corni. |
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