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| Effects of active extractions from Chinese medicines on the proliferation of neural stem cells cultured in vitro and related mechanisms study of proliferation |
| Hits 1379 Download times 1890 Received:January 02, 2009 |
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| DOI
10.11656/j.issn.1672-1519.2009.02.30 |
| Key Words
embryonic neural stem cells;proliferation;ginsenoside Rg1;astragaloside IV;cyclin D1;Hes1;Hes5 |
| Author Name | Affiliation | | Ishida Torao | Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, Suzuka 510-0293, Japan | | WANG Xiu-yun | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | CHAI Li-juan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | 周志焕 | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | 钟佩茹 | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] To explore the effective Chinese medicine extractions that have the ability of protecting neural stem cells (NSCs). Rat embryonic were cultured in vitro. The proliferation of NSCs was observed and identified. Different Chinese medicine extractions were applied,whose effect and function mechanism were investigated. [Methods] The NSCs cultures were generated from the brain of embryonic day (E) 16 SD rat. Primitive NSCs were cultured,proliferated and passaged. The NSCs were identified by the immunocyto-chemical (ICC) staining of Nestin. The ICC staining of BrdU was adopted to characterize the proliferation of NSCs. According to limited dilution method,the effect of different Chinese medicine extractions on the proliferation of NSCs was observed,such as Ginsenoside Rg1 and AS-tragaloside IV. [Results] The NSCs were successfully cultured and proliferated in vitro. Different dosages of Ginsenoside Rg1 and As-tragaloside IV couldpromote the proliferation of NSCs in vitro. Compared with the control group,the numbers of nerurospheres of the three dosage Ginsenoside Rg1 groups (40,4,0.4 μM) were increased obviously (P<0.05,P<0.01). The neurospheres numbers of all of three Astragaloside IV Groups (6,0.6,0.06 μM) were increased significantly(P<0.05,P<0.01). The results showed that the lower dosage groups of both medicines have more outstanding effects than other groups. Real time RT-PCR analysis of Hes1,Hes5 and cyclinD1 deemonstrated that the Hes1 expression of high dosage group of Ginsenoside Rg1 (40 μM) and Hes5 expression of high dosage group of Astragaloside IV (6 μM) increased significantly (P<0.05). Simultaneously,Hes5 expression of middle dosage froup of Ginsenoside Rg1 (4 μM) increased slightly (P>0.05). CyclinD1 expression increased in different dosage groups of Ginsenoside Rg1 and Astragaloside IV (P>0.05). [Conclutions] Ginsenoside Rg1 and Astragaloside IV significantly promote the proliferation of NSCs in vitro. The real-time quantitative data suggest that Hes1,Hes5 and cyclinD1 might be involved in the proliferation of NSCs induced by Ginsenoside Rg1 and Astragaloside IV. |
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