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| Apoptosis-inducing effect of Ling-Qi capsule contained serum on K562 leukemia cells |
| Hits 1606 Download times 1500 Received:October 20, 2008 |
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| DOI
10.11656/j.issn.1672-1519.2009.03.27 |
| Key Words
Ling-Qi Capsule;serum-pharmacology;K562 cell line;Apoptosis |
| Author Name | Affiliation | | ZHAO Da-zhuo | Heilongjiang University of TCM, Harbin 150040, China | | DU Yong-qiang | Heilongjiang University of TCM, Harbin 150040, China | | BAI Yun | Heilongjiang University of TCM, Harbin 150040, China | | 姜波 | Heilongjiang University of TCM, Harbin 150040, China | | 苏云明 | 黑龙江中医药大学, 哈尔滨, 150040 |
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| Abstract
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| [Objective] To study the effect of the herb-contained serum of Ling-Qi Capsule(LQC) on the proliferation and apoptosis of K562 leukemia cells in vitro. [Methods] The herb serum contained LQC was prepared with serologic pharmacological method and the K562 leukemia cells was treated with it in different concentration. The effect of the serum contained LQC on apoptosis of K562 cells was detected by flowcytometry and DNA agarose gel electrophoresis. [Results] The herb-contained serum in different concentration could inhibit the proliferation of K562 leukemia cells directly in a dose-dependent pattern. It could increase the apoptosis rate of the K562 cells compared with blank control group as increasing of drug dose(P<0.01). The DNA ladder was also observed by agarose gel electrophoresis. [Conclusion] The LQC contained serum can inhibit the proliferation,increase the differentiation and induce the apoptosis of K562 leukemia cells in a dose and time dependent manner. So we can conclude that cytotoxic action of the serum contained LQC may be related to the effect of inducing the apoptosis. |
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