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| Multi-target screening of traditional Chinese medicine with regulating glucose and lipid metabolism disorder |
| Hits 1491 Download times 1655 Received:December 04, 2008 |
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| DOI
10.11656/j.issn.1672-1519.2009.03.34 |
| Key Words
hepatoma cell line Hep-G2;glucose and lipid metabolism-related genes;Real Time RT-PCR |
| Author Name | Affiliation | | CUI Guang-zhi | Tianjin University of TCM, Tianjin 300193, China | | LI Hui-ying | Tianjin University of TCM, Tianjin 300193, China | | ZHANG Yan-jun | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To observe the effect of several tradition Chinese medicines on the glucose and lipid metabolism-related genes of human hepatoma cell line Hep-G2,including cell angiopoietin-related growth factor(AGF),glucose-6-phosphatase(G-6-Pase),low density lipoprotein receptor(LDLR) and insulin receptor substrate-2(IRS-2). [Methods] The human hepatoma cell line Hep-G2 was cultured and the impaction of the components of Chinese medicine on AGF,G-6-Pase,LDLR and IRS-2 mRNA expression was detected by real time RT-PCR. [Results] Berberine can improve the AGF and LDLR mRNA expression,and curcumin can reduce the G-6-Pase mRNA expression of Hep-G2 cell line. [Conclusion] The berberine and curcumin have some certain therapeutic effect on diabetes through regulating the related genes with glucose and lipid metabolism. |
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