|
| Effect of Qi supplement and Blood activation therapy on treating TEMT in UUO rats |
| Hits 1577 Download times 1673 Received:April 23, 2009 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2009.04.24 |
| Key Words
Qi supplement and Blood activation therapy;tubular epithelial myofibroblast transdifferentiation;TGF-β1;Smad2/3;α-SMA |
| Author Name | Affiliation | | LIU Li | Department of Anatomy and Histology, Capital University of Medical Sciences, Beijing 100069, China | | LIU Wen-jun | Guanganmen Hospital of China Academy of TCM, Beijing 100053, China | | YANG Lin | Department of Anatomy and Histology, Capital University of Medical Sciences, Beijing 100069, China | | 景鹏 | Department of Anatomy and Histology, Capital University of Medical Sciences, Beijing 100069, China | | 杨连雪 | Department of Anatomy and Histology, Capital University of Medical Sciences, Beijing 100069, China | | 刘娜 | Department of Anatomy and Histology, Capital University of Medical Sciences, Beijing 100069, China |
|
| Abstract
|
| [Objective] To investigate the regulating effect of qi supplement and blood activation therapy method in TCM on expression of the TGF-β1, Smad2/3 and α-SMA in rats with unilateral ureteral obstruction and to explore the therapeutic action on tubular epithelial myofibroblast transdifferentiation. [Methods] The unilateral ureteral ligation was used to induce renal interstitial fibrosis in rats. They were divided into treatment groups and sham operation group with 5 rats in each. Thirty male rats were selected and randomly divided into the model group, the high, middle and low dose TCM groups, Western medicine group. At 14th postoperative day, the nephridial tissues of operating side were harvested to detect the change of pathology. The expressions of TGF-β1, Smad2/3 and α-SMA in renal tissue were examined by using immunohistochemistry method. [Results] The Qi supplement and blood activation therapy could lessen the renal interstitial fibrosis and down-regulate the expression of TGF-β1, Smad2/3 and α-SMA. [Conclusion] The Qi supplement and blood activation therapy can inhibit the tubular epithelial myofibroblast transdifferentiation by down-regulating the expression of TGF-β1, Smad2/3 and αSMA. |
|
|
|
|
|
|
|
