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Effect of caulis sargentodoxae prescription on the growth of endometrial cells cultured in vitro in rats
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DOI   10.11656/j.issn.1672-1519.2009.04.29
Key Words   caulis sargentodoxae prescription;cell culture;seropharmacology;drug-containg serum;endometriosis
Author NameAffiliation
LIU Jin-hui Tianjin Public Security Hospital, Tianjin 300400, China 
ZHANG Ting-ting The Affiliated Shuguang Hospital of Shanghai University of TCM, Shanghai 200021, China 
SHU Lan-di The Affiliated Shuguang Hospital of Shanghai University of TCM, Shanghai 200021, China 
戴德英 The Affiliated Shuguang Hospital of Shanghai University of TCM, Shanghai 200021, China 
曹霖 Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China 
Abstract
    [Objective] To study the effect of Caulis Sargentodoxae prescription on the growth of endometrial cells cultured in vitro in rats. [Methods] Female Sprague-Dawley rats were collected to establish the model of endometriosis in vitro. After culture in media with different concentration Caulis Sargentodoxae prescription and Danazol drug-containing serum for 24, 48, 72 and 96 h, the cell viability was determined by MTT assay. [Results] After different culture periods in different concentration media with drug-containing rat serum, cell viability cultured with drug-containing serum was lower than control cells timeand dosadependently. [Conclusion] Caulis Sargentodoxae prescription and Danazol can restrain the growth of rat endometrial cells.

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