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| Effects of Wu Yu decoction drug-containing serum on expression of INHB,FSHR mRNA in ovarian granulose cell and secretion function |
| Hits 1501 Download times 1628 Received:December 26, 2009 |
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| DOI
10.11656/j.issn.1672-1519.2010.02.29 |
| Key Words
Wu Yu decoction;ovarian granulose cell;INHB;FSHR mRNA;E2 |
| Author Name | Affiliation | | LI LING | Clinical College of Beijing University of Chinese Medicine, Beijing 100029, China | | HUANG Wen-ling | Department of Gynecology, Dong Fang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China | | JIN Zhe | Department of Gynecology, Dong Fang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China | | 林佳静 | Clinical College of Beijing University of Chinese Medicine, Beijing 100029, China | | 蔡品均 | Clinical College of Beijing University of Chinese Medicine, Beijing 100029, China |
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| Abstract
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| [Objective] To research the effects of Wu Yu decoction drug-containing serum on the expression of INHB,FSHR mRNA in ovarian granulose cell (abbr.GC) and the secretion function in rats. [Methods] Wu Yu decoction,FSH drug-containing serum and the normal rat serum were added into rat ovarian GC culture medium system. They were cultured for 48 hours. The expression of INHB was measured by ELISA,FSHR mRNA expression levels by RT-PCR,E2 levels of each group by radioimmunoassay. [Results] The E2 level was significantly higher in treatment groups than that in control groups,and the expression of INHB,FSHR mRNA significantly increased,among them medium dosage group of Wu Yu decoction was superior than that in Western medicine groups. [Conclusion] Wu Yu decoction drug-containing serum promotes the expression of INHB,FSHR mRNA and secretion of E2 of ovarian GC significantly. It is proved that Wu Yu decoction can enhance the ovarian reaction function to gonadotropins and improve the HPOA through adjusting internal factors of ovary and enhancing the expression of FSHR mRNA,thereby to promote the development and maturation of follicular and ovulation. |
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