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| Establishment of the cell suspension culture system of periploca sepium bunge and content measurement of the effective constituent of periplocin and 4-methoxysalicylaldehyde |
| Hits 1810 Download times 1798 Received:October 20, 2009 |
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| DOI
10.11656/j.issn.1672-1519.2010.02.37 |
| Key Words
periploca sepium bunge;system of cell suspension culture;minimal medium;periplocin;4-methoxysalicylaldehyde |
| Author Name | Affiliation | | ZHANG Jian | Tianjin University of TCM, Tianjin 300193, China | | GAO Wen-yuan | Tianjin University of TCM, Tianjin 300193, China
Tianjin University of Science and Technology, Tianjin 300457, China | | WANG Juan | 天津大学药物科学与技术学院 天津 300072 | | 赵志勇 | 天津大学药物科学与技术学院 天津 300072 |
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| Abstract
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| [Objective] To establish the Cell Suspension Culture System of Periploca sepium Bunge and measure the constituent of Periplocin and 4-Methoxy salicylaldehyde. [Methods] Cut and collected the root and induce callus under sterile conditions. Then,propagate the callus for several cycles. Select eugonic and incompact callus and inoculate them to liquid medium to establish the System of Cell Suspension Culture. Then,measure the content of Periplocin and 4-Methoxysalicylaldehyde in cell. [Results] The Cell Suspension Culture System of Periploca sepium bunge was established and the constituent of periplocin and 4-Methoxysalicylaldehyde was measured as 4.06 μg/g and 1.92 μg/g-respectively. [Conclusion] The Cell Suspension Culture System of Periploca sepium Bunge can be established successfully by tissue culture,but the effective constituent of the cell was low,so it was necessary to study the minimum medium and culture conditions further. |
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