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Study on the Measurement and Methodology of 1-deoxynojirimycin in Total Alkaloids from Mulberry Leaf |
Hits 1807 Download times 2005 Received:November 21, 2009 |
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DOI
10.11656/j.issn.1672-1519.2010.02.38 |
Key Words
mulberry leaf;1-deoxynojirimycin in total alkaloids;pre-column derivation |
Author Name | Affiliation | LIU Wei-jun | Tianjin University of TCM, Tianjin 300193, China | ZHAO Jun | 天津中医药大学 天津 300193 | ZENG Sen | 天津中医药大学 天津 300193 | 刘美平 | 天津中医药大学 天津 300193 |
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Abstract
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[Objective] To extract and purify 1-deoxynojirimycin in total alkaloids from mulberry leaf and to establish the method of content determination. [Methods] The 1-deoxynojirimycin in total alkaloids from mulberry leaf was measured by using pre-column derivation high performance liquid chromatography. [Results] In this way,the purity was exceeded above 7.99%,8.64%,7.09%. [Conclusion] This method was simple,high-speed,and accurate. |
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