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Study on the Measurement and Methodology of 1-deoxynojirimycin in Total Alkaloids from Mulberry Leaf
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DOI   10.11656/j.issn.1672-1519.2010.02.38
Key Words   mulberry leaf;1-deoxynojirimycin in total alkaloids;pre-column derivation
Author NameAffiliation
LIU Wei-jun Tianjin University of TCM, Tianjin 300193, China 
ZHAO Jun 天津中医药大学 天津 300193 
ZENG Sen 天津中医药大学 天津 300193 
刘美平 天津中医药大学 天津 300193 
Abstract
    [Objective] To extract and purify 1-deoxynojirimycin in total alkaloids from mulberry leaf and to establish the method of content determination. [Methods] The 1-deoxynojirimycin in total alkaloids from mulberry leaf was measured by using pre-column derivation high performance liquid chromatography. [Results] In this way,the purity was exceeded above 7.99%,8.64%,7.09%. [Conclusion] This method was simple,high-speed,and accurate.

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