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| Effect of preventive acupuncture and preventive moxibustion at Guanyuan point on the expression of HSP70 and HSP70 mRNA in hypothalamus of ovariectomy rats |
| Hits 1719 Download times 1568 Received:December 15, 2009 |
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| DOI
10.11656/j.issn.1672-1519.2010.03.14 |
| Key Words
preventive acupuncture and preventive moxibustion;menopause;hypothalamus;Heat Shock Protein70 |
| Author Name | Affiliation | | WANG Hong-bin | College of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, Beijing 100029, China | | LI Xiao-hong | College of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, Beijing 100029, China | | Mo Jie | College of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, Beijing 100029, China | | 孙志芳 | College of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, Beijing 100029, China | | 王婧 | College of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, Beijing 100029, China | | 何龙 | College of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, Beijing 100029, China | | 张露芬 | College of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, Beijing 100029, China | | 解秸萍 | College of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, Beijing 100029, China |
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| Abstract
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| [Objection] To investigate the influence of preventive acupuncture and preventive moxibustion at Guanyuan(CV4) point on the function of hypothalamus in ovariectomy rats.[Methods] Thirty female SD rats,aged 3.5 months,were randomly divided into normal group,sham operation group,ovariectomy model group,preventive acupuncture group and preventive moxibustion group.In preventive acupuncture and preventive moxibustion groups,Guanyuan was punctured with the needle retained for 20 min,and given with moxibustion for one moxa-cone respectively from aged 3.5th month.The treatment was conducted twice every week,4 weeks altogether.At 4.5th month,ovariectomy was made in the preventive acupuncture group,the preventive moxibustion group and the ovariectomy model group.For the sham operation group,only a little fat around the ovary was removed without ovariectomy,and nothing was not applied to the normal group.Immunohistochemical and in situs hybridization methods were used to investigate changes of expressions of HSP70 and HSP70 mRNA in arcuate nucleus,paraventricular nucleus and supraoptic nucleus.[Results] Compared to the normal group and sham operation group,the expressions of HSP70 and HSP70 mRNA in arcuate nucleus,paraventricular nucleus and supraoptic nucleus of hypothalamus increased in the ovariectomy model group,preventive acupuncture group and preventive moxibustion group.After preconditioning with acupuncture or moxibustion,the expression of HSP70 and HSP70 mRNA decreased,the expression of HSP70 mRNA in arcuate nucleus significantly decreased in preventive acupuncture group and preventive moxibustion group(P<0.01).[Conclusion] Preventive acupuncture and moxibustion at Guanyuan point have an effect on the expressions of HSP70 and HSP70 mRNAin different hypothalamic nuclei in ovariectomy rats,which possibly is one way for modulating the menopausal dysfunction. |
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