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| NMR-spectroscopy-based metabonomic study on the mouse model of blood deficiency syndrome induced by radiation |
| Hits 1738 Download times 1908 Received:January 30, 2010 |
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| DOI
10.11656/j.issn.1672-1519.2010.03.29 |
| Key Words
radiation;Siwutang;blood deficiency;metabonomics;NMR |
| Author Name | Affiliation | | HUO Chao | Beijing Institute of Radiation Medicine, Beijing 100850, China | | WANG Mu | Beijing Institute of Radiation Medicine, Beijing 100850, China | | MA Zeng-chun | Beijing Institute of Radiation Medicine, Beijing 100850, China | | 张琪 | 军事医学科学院生物医学分析中心, 北京, 100850 | | 颜贤忠 | 军事医学科学院生物医学分析中心, 北京, 100850 | | 张伯礼 | 天津中医药大学, 天津, 300193 | | 高月 | Beijing Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract
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| [Objection] To explore the essence of blood deficiency syndrome and the mechanism of the effects of Siwutang based on the characteristic changes in metabolic profiles of C57 mice with blood deficiency induced by radiation before the treatment of Siwutang.[Methods] Thirty C57 mice were randomly divided into three groups of 10 animals:the control group and two blood deficiency model groups without or with Siwutang treatment.The animals were sacrificed the 7th day after radiation and the blood,spleen,thymus and bone marrow were obtained.The metabolic profiles of serum,aqueous and lipidic extracts of thymus,spleen and bone marrow were investigated using 1H nuclear magnetic resonance spectroscopy(NMR).The NMR spectra were integrated in segments of 0.01 ppm,normalized and then analyzed using SIMCA-P software to visualize the similarities and differences in metabolic profiles between these groups.[Results] Compared with the control group,the model group contained different levels of lactate,choline,glucose,taurine,alanine,LDL,glycerin,UFA,creatinine,histidine,valine/leucine/isoleucine.In the Siwutang treated group,the above-mentioned changes were reversed.[Conclusion] The injury established by radiation was a kind of proper model to develop further metabonomics researches.The disorder of energy metabolism and osmoregulation,and the damage of immune function were observed by NMR-based-metabonomics method.Siwutang,the classic complex for treating blood deficiency in clinic,could improve the immune function,accommodate energy metabolism and stabilize cellular membrane. |
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