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Research on the making method of medicine serum contained LiuShen pill
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DOI   10.11656/j.issn.1672-1519.2010.03.37
Key Words   LiuShen pill;HL-60 cell;Chinese herbs serum pharmacology
Author NameAffiliation
HUANG Zhen-dong Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Science, Tianjin 300020, China 
DAI Xi-meng Tianjin University of TCM, Tianjin 300193, China 
Guo Yi Tianjin University of TCM, Tianjin 300193, China 
Abstract
    [Objection] To study the making method of medicine serum of LiuShen pill,to explore serum pharmacology method of LiuShen pill.[Methods] To produce medicine serum of Liu Shen pill with different dose,different time of pouring medicine,different time of blood sampling.To measure content of arsenic in serum with atomic absorption method,to qualitative analysis toadfish in medicine serum of Liu Shen pill with thin layer chromatography.MTT make certain the best scheme of making method of medicine serum of Liu Shen pill.[Results] Content of arsenic in serum raises with dose adding,time of pouring medicine and time of blood sampling prolonging.Thin layer chromatography indicate that there was toadfish in medicine serum of Liu Shen pill.The suppressive rate of medicine serum of Liu Shen pill.On HL-60 cell raises with adding of dose and time of pouring medicine and prolonging of blood sampling and dose of medicine serum of Liu Shen pill.[Conclusion] To use the corresponding scheme of producing medicine serum for different traditional Chinese medicine.

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