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| Proliferating activity of peritoneal macrophage regulated by Rhubarb combined with Huoxue or Zhixue Drug |
| Hits 1520 Download times 1737 Received:March 08, 2010 |
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| DOI
10.11656/j.issn.1672-1519.2010.04.27 |
| Key Words
abdominal macrophage;MTT colorimetric assay;Chinese medicine herbs |
| Author Name | Affiliation | | LI Ji-kun | Tianjin Institute of Acute Abdominal Disease of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China | | ZHANG Yan-ping | Tianjin Institute of Acute Abdominal Disease of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China | | WANG Qian. | Tianjin Institute of Acute Abdominal Disease of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China | | 薛小平 | Tianjin Institute of Acute Abdominal Disease of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China | | 杨秀竹 | Tianjin Institute of Acute Abdominal Disease of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China |
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| Abstract
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| [Objective] To observe the compatibility of rhubarb with salvia miltiorrhiza,compound salvia miltiorrhiza and Bletilla striata and the regulatory effect of them on the abdominal macrophage proliferation activity. [Methods] The method of isolation,purification of abdominal macrophage was established.Using MTT colorimetric assay(MCA) the regulatory effect of the four compatible drugs on the macrophages activity was measured with three different concentrations and three sequential time points. [Results] Rhubarb integrated with Bletilla striata at high,middle concentration could inhibit the macrophages activity(P<0.05).On the contrast,Rhubarb with salvia miltiorrhiza at high,middle and low concentration could enhance the proliferation of abdominal macrophage.Rhubarb with compound Salvia miltiorrhiza had an enhancing effect only at high or middle concentration(P<0.01 or P<0.05). [Conclusion] The experimental results in vitro showed that Rhubarb combined with different Chinese crude drugs,can produce significantly different effect on the proliferation activity of macrophage,thus providing some data for further research of Chinese drugs compatibility. |
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