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| Determination of effective content of polygonum multiflorum in Guzhiwan Ⅱ |
| Hits 2165 Download times 2183 Received:April 22, 2010 |
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| DOI
10.11656/j.issn.1672-1519.2010.04.35 |
| Key Words
Guzhiwan;HPLC;polygonum multiflorum;effective content;2,3,5,4,-tetrahydroxy diphenyl ethylene-2-0-β-D-glucoside |
| Author Name | Affiliation | | XUE Ai-hua | Nankai TCM Hospital in Tianjin, Tianjin 300102, China | | SONG Wen-jing | Nankai TCM Hospital in Tianjin, Tianjin 300102, China |
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| Abstract
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| [Objective] To establish the determination method of the effective content of 2,3,5,4,-tetrahydroxy diphenyl ethylene-2-0-β-Dglucoside of polygonum multiflorum in GuzhiwanⅡ. [Methods] HPLC,agilent column;mobile phase:acetonitrile-water(17:83);detection wave length:320 nm;column temperature:40℃;the flow rate:1.0 mL/min. [Results] The liner range was 0.063～0.7875 μg(r=0.999 9),the recovery rate was 101.37% and RSD was 1.76%. [Conclusion] The method was easy manipulated and accurate with a good reproducibility.It can be used to determine the content of 2,3,5,4,-tetrahydroxy diphenyl ethylene-2-0-β-D-glucoside. |
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