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| Quantitative analysis of polysaccharide in radix bupleuris from different areas by ultraviolet visible spectrophotometry |
| Hits 1744 Download times 2098 Received:April 12, 2010 |
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| DOI
10.11656/j.issn.1672-1519.2010.04.36 |
| Key Words
Polysaccharide;quantitative analysis;ultraviolet visible spectrophotometry |
| Author Name | Affiliation | | LI Di-hua | Tainjin Nankai Hospital, Institute of Acute Abdomen by Integrated Medicine, Tianjin 300100, China | | LIU Jun-hong | Tainjin Nankai Hospital, Institute of Acute Abdomen by Integrated Medicine, Tianjin 300100, China | | WU Xiao-xian | Tainjin Nankai Hospital, Institute of Acute Abdomen by Integrated Medicine, Tianjin 300100, China |
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| Abstract
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| [Objective] To set up an ultraviolet visible spectrophotometry(UV) method to determine the content of polysaccharide in herbs obtaining from different areas. [Methods] The content of Polysaccharide was tested with the color appearing in the phenol-sulphuricacid reagent.The testing wavelength was 478nm.Its UV assaying method was found by experimenting on the key steps and methodology of extraction. [Results] UV assay method for Polysaccharide was simple and convenient,accurate,and stable.Its linearity range was 10.484～75.936μg.Among the Radix bupleuris from 11 places of origin,the content range of Polysaccharide per 1 gram of crude drug was 46.99～96.965mg. [Conclusion] The method was simple and easy to perform and the obtained results were stable and reliable. |
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